Since 2D Experiments are more difficult to set up, than 1D Experiments, every user should be
extremely cautious when attempting to start the procedure.
The preparation of the experiments requires a lot of interaction of the user with the spectrometer.
If you are not 100% certain about the procedure, you should ask the NMR Specialist for assistance. Otherwise you might do serious damage to the equipment.

All 2D experiments can be broken down into 4 steps:
    I.     Preparing the 1D projections (These will be used when you plot the 2D spectra.)
    II.    Preparing and starting the 2D experiment (Enter all parameters for the 2D experiment.)
    III.  Processing the 2D data set.
    IV.  Plotting the 2D spectrum.

The following is a list of experiments which are currently available and will be continuously expanded:
(The substrate requirements show the amount of sample needed, to obtain a clean spectrum
in the indicated time period. A molecular weight of 500 is assumed.)
 

1. H,H COSY: to identify protons, which couple with each other

    Substrate requirement: 5 - 10 mg,      30 minutes
2. H,H NOESY: to identify the distance between different protons

    Substrate requirement: 10 - 20 mg,     4 hours
3. H,H TOCSY: to see the complete coupling networks between protons

    Substrate requirement: 5 - 10 mg,       1 hour
4. J-resolved H,H correlation: to separate overlapping ¹H multiplets

    Substrate requirement: 5 - 10 mg,       1 hour
5. C,H Correlation: to identify directly connected protons and carbon atoms

    Substrate requirement: 50 - 100 mg,    1 hour
6. COLOC: long range (2,3 bonds) C,H correlation

LOCOF1: same as COLOC, plus: long range C,H couplings resolved in F1
    Substrate requirement: 50 - 100 mg     overnight
7. J-resolved C,H Correlation: to separate overlapping ¹³C multiplets

    Substrate requirement: 50 - 100 mg     1 hour
8. C,C INADEQUATE: to identify directly connected carbon atoms

    Substrate requirement: >1000 mg        >24 hours

    All the instructions have to be followed precisely and in the order in which they are listed!
 

1. Record a routine Proton NMR Spectrum. Process it as usual (Fourier transformation, phase correction, baseline correction, calibration).
2. Set CX = 15, CY = 15, MAXY = 17.
3. Write the spectrum to the disk. (Use only numbers in the extension, no letters!)

(You have to save it NOW, NOT later!)
4. Enter the EP mode and press "Ctrl-R" to display the complete spectrum.
• Set the cursor in front of the first signal in the spectrum (you may ignore a solvent signal, if it is far away from the signals of your compound.)
• Press "R" to define the start of the relevant spectrum.
• Move the cursor behind the last signal in the spectrum and press "R" again to define the end. Now the display should show only the desired region.

Be careful, not to exclude any signals from your compound!
• Do NOT press "enter", or you will have to repeat step 4.
• At this point press "Ctrl-O". This will leave the EP mode and display the changed values for O1 and SW. Type SW« and increase its value by approx. 20%.
5. Write down O1, SW and SR, you will need them later.
6. This spectrum must not be stored to disk, as it is useless now!!
7. Repeat steps 1 to 5 for Carbon-13, you should change CX to 18 in step 2.

(The C-13 projection is only necessary for those 2D experiments, which involve the C-13 nucleus like the C,H correlations and the INADEQUATE experiment.)
8. Continue with the preparation of the individual 2D experiments.

ONLY use the procedure, which describes YOUR experiment. Please follow it exactly!
Now you will need your list with the O1, SW and SR values from step C.I.5.

C.IIa     H,H COSY

1. Read your PROTON projection: RE filename.extn « PJ filename.extn «
2. Set the following parameters:

    NOBC = 0,     SI = 1K,        ND0 = 1,  MC2 = M,     RG = 8,
    WDW1 = S,   WDW2 = S, SSB1 = 3,    SSB2 = 3,
    SR1 and SR2 = the SR from the proton spectrum.
    O1 = O2 = O11 = the O1 you found for the proton spectrum.
    SW2 = the SW you found for the proton spectrum, SW1 = half of SW2.
3. Set up the COSY experiment: AS COSYDQF.AU «

This will display the complete microprogram and then (maybe after a few " « ") it will print out the parameters it needs on the LCD display or on the screen.
You may accept them by hitting return or you may change them one by one by entering your new values. Recommended are:
    D1 = 2             P1 = 12.5         D0 = 3U         D2 = 50M
    D3 = 3U          RD = 0             PW = 0           DE must not be changed!
    NS = 16          DS = 2             NE = 256        IN must not be changed!
Please keep in mind that you must not press " « " after pressing a letter key!
If you do so by mistake, press Ctrl-Q and repeat step 3.
4. Type ST2D « to bring up the 2D parameter screen.
5. Adjust SI and NE simultaneously (only values of 2ⁿ are allowed here) and repeat

step 4 until the resolution (Hz/Pt) is between 3 and 6.
6. If you need a better resolution, there is a better experiment.(Ask the NMR specialist.)
7. Type EXPT« to display the duration of the experiment.

If the duration is too long, decrease D1 (but not below "1") or NE (decrease not by more than 25%, even numbers only) and check the duration again until it is acceptable.
Do not type ST2D after changing NE!!
You may also change NS to 8, if the sample is concentrated enough (>10 mg) or to multiples of 16 if the sample is very dilute (<2mg).
8. Check I2D, if it is not = 1.0 ±2% then you must start over from step 1!!
9. Check SF1 and SF2. If they are not equal to the proton frequency, start over from step 1!!
10. Write the parameters to the disk: WJ2D filename.2DP «
11. Turn off sample spinning and increase the "LOCK GAIN" to the top edge of the screen.
12. Start the experiment: AU COSYDQF.AU «

    Enter the requested filename. The extension ".SER" is required!!
13. Watch the FID during the first scan. If it is too big (±1 box from the center) or too small, press "Ctrl-H", change the setting for RG and restart the experiment with AU«.

1. Read your PROTON projection: RE filename.extn «     PJ filename.extn «
2. Set the following parameters:

    SI = 1K,      ND0 = 2,         MC2 = W,    REV = Y,     REDF = N,
    WDW1 = S,   WDW2 = S, SSB1 = 3,      SSB2 = 3,  RG = 8,      NOBC = 1
    O1 = O2 = O11 = the O1 you found for the proton spectrum.
    SR1 and SR2 = the SR you found for the proton spectrum.
    SW2 = the SW you found for the proton spectrum, SW1 = half of SW2.
3. Set up the NOESY experiment: AS NOESYPH.AU «

This will display the complete microprogram and then (maybe after a few " « ") it will print out the parameters it needs on the LCD display or on the screen.
You can change them one by one by entering the new values. Recommended are:
    D1 = 2         P1 = 12.5         D0 = 3U         P2 = 12.5
    D9 = 2         P3 = 12.5         RD = 0           PW = 0         DE must not be changed!
    NS = 16      DS = 2              NE = 512       V9 = 1          IN must not be changed!
Please keep in mind that you must not press " « " after pressing a letter key!
If you do so by mistake, press Ctrl-Q and repeat step 3.
4. Type ST2D « to bring up the 2D parameter screen.
5. Adjust SI and NE simultaneously (only values of 2ⁿ are allowed here) and repeat step 4 until the resolution (Hz/Pt) is between 3 and 6.
6. Type EXPT« to display the duration of the experiment.
7. If the duration is too long, decrease D1 (but not below "1") or NE (decrease not by more than 25%, even numbers only) and check the duration again until it is acceptable.

Do not type ST2D after changing NE!!
It is not advisable to change NS, but if you do, then only in multiples of 8.
8. Check I2D, if it is not =1.000 then you must start over from step 1!!
9. Check SF1 and SF2. If they are not equal to the proton frequency, start over from step 1!!
10. Write the parameters to the disk: WJ2D filename.2DP «
11. Turn off the sample spinning and increase the "LOCK GAIN" until the lock signal is at the top edge of the screen.
12. Start the experiment: AU NOESYPH.AU «

    Enter the requested filename. The extension ".SER" is required!!
13. Watch the FID during the first scan. If it is too big (±1 box from the center) or too small, press "Ctrl-H", change the setting for RG and restart the experiment with AU«.

    Switch the spectrometer to inverse operation mode. (Ask the NMR specialist for help.)

1. Read your PROTON projection: RE filename.extn « PJ filename.extn «
2. Set the following parameters:

    SI = 2K,      ND0 = 2,         MC2 = W,       REV = Y,   REDF = N,
    WDW1 = S,   WDW2 = S, SSB1 = 6,         SSB2 = 6,     RG = 8,     NOBC = 1
    O1 = O2 = O11 = the O1 you found for the proton spectrum.
    SR1 and SR2 = the SR you found for the proton spectrum.
    SW2 = the SW you found for the proton spectrum, SW1 = half of SW2.
3. Set up the TOCSY experiment: AS TOCSY2D.AU «

This will display the complete microprogram and then (maybe after a few " « ") it will print out the parameters it needs on the LCD display or on the screen.
You can change them one by one by entering the new values. Recommended are:
    D1 = 2         S1 = 8H         P1 = 38         D0 = 3U         P3 = 2500
    P2 = 76        L6 = 50         P4 = 2500     RD = 0           PW = 0
    DE must not be changed!    NS = 16        DS = 2           NE = 1024
    IN must not be changed!
Please keep in mind that you must not press " « " after pressing a letter key!
If you do so by mistake, press Ctrl-Q and repeat step 3.
4. Type ST2D « to bring up the 2D parameter screen.
5. Adjust SI and NE simultaneously (only values of 2ⁿ are allowed here) and repeat step 4 until the resolution (Hz/Pt) is between 3 and 6.
6. Type EXPT« to display the duration of the experiment.
7. If the duration is too long, decrease D1 (but not below "1") or NE (decrease not by more than 25%, even numbers only) and check the duration again until it is acceptable.

Do not type ST2D after changing NE!!
8. It is not advisable to change NS, but if you do, then only in multiples of 8.
9. Check I2D, if it is not =1.000 then you must start over from step 1!!
10. Check SF1 and SF2. If they are not equal to the proton frequency, start over from step 1!!
11. Write the parameters to the disk: WJ2D filename.2DP «
12. Turn off sample spinning and increase the "LOCK GAIN" to the top edge of the screen.
13. Start the experiment: AU TOCSY2D.AU «

    Enter the requested filename. The extension ".SER" is required!!
14. Watch the FID during the first scan. If it is too big (±1 box from the center) or too small,

press "Ctrl-H", change the setting for RG and restart the experiment with AU«.

1. Read your PROTON projection: RE filename.extn « PJ filename.extn «
2. Set the following parameters:

    SI = 4K,      ND0 = 2,         MC2 = M,    REV = N,     REDF = N,
    WDW1 = S,   WDW2 = S,  SSB1 = 4,     SSB2 = 4,  RG = 8,       NOBC = 0
    O1 = O2 = O11 = SR1 = the O1 you found for the proton spectrum.
    SR2 = the SR you found for the proton spectrum.
    SW2 = the SW you found for the proton spectrum, SW1 = "30.0".
3. Set up the J-resolved experiment: AS JRES.AU «

This will display the complete microprogram and then (maybe after a few " « ")it will print out the parameters it needs on the LCD display or on the screen.
You can change them one by one by entering the new values. Recommended are:
    D1 = 2              P1 = 12.5            D0 = 3U             P2 = 25.0
    RD = 0             PW = 0                DE must not be changed!
    NS = 16           DS = 2                 NE = 64             IN must not be changed!
Please keep in mind that you must not press " « " after pressing a letter key!
If you do so by mistake, press Ctrl-Q and repeat step 3.
4. Type ST2D « to bring up the 2D parameter screen.

Adjust SI (only values of 2ⁿ are allowed here) and repeat step 4 until the resolution (Hz/Pt) in the F2 dimension is between 0.5 and 1.5.
5. Type ST2D « and then type EXPT« to display the duration of the experiment.

If the duration is too long, decrease D1 (but not below "1") or NE (decrease not by more than 25%, even numbers only) and repeat step 5 until the duration is acceptable.
It is not advisable to change NS, but if you do, then only in multiples of 8.
6. Type I2D « and enter the 2ⁿ-number closest to the displayed value.

(i.e. set it to . . ., 0.25, 0.5, 1, 2, 4, 8, 16, . . .; only 2ⁿ-values are allowed!)
7.  If you want to improve the resolution: double SI2 and set SI1 to "256W".
8. Check SF1 and SF2. If they are not equal to the proton frequency, start over from step 1!!
9. Write the parameters to the disk: WJ2D filename.2DP «
10. Turn off the sample spinning and increase the "LOCK GAIN" until the lock signal is at the top edge of the screen.
11. Start the experiment: AU JRES.AU «

    Enter the requested filename. The extension ".SER" is required!!
12. Watch the FID during the first scan. If it is too big (±1 box from the center) or too small, press "Ctrl-H", change the setting for  and restart the experiment with AU«.

1. Read your CARBON-13 projection: RE filename.extn « PJ filename.extn «
2. Set the following parameters:

    SI = 1K,      ND0 = 2,         MC2 = M,        REV = N,      REDF = N,
    WDW1 = S,   WDW2 = S,  SSB1 = 4,         SSB2 = 4,         NOBC = 0
    O2 = O11 = the O1 you found for the proton spectrum.
    O1 = the O1 you found for the carbon spectrum.
    SR1 = the SR you found for the proton spectrum.
    SR2 = the SR you found for the carbon spectrum.
    SW1 = half of the SW you found for the proton spectrum.
    SW2 = the SW you found for the carbon spectrum.
3. Set up the C,H correlation experiment: AS XHCORRD.AU «

This will display the complete microprogram and then (maybe after a few " « ")it will print out the parameters it needs on the LCD display or on the screen.You can change them one by one by entering the new values. Recommended are:
    D1 = 2         S1 = 0H         P1 = 10.4         D0 = 3U         D3 = 3.4M
    P2 = 21       P4 = 10.4       P3 = 5.2           D4 = 1.7M      S2 = 16H (careful !!)
    RD = 0        PW = 0          DE must not be changed!
    NS = 32      DS = 0           P9 = 80            NE = 128        IN must not be changed!
Please keep in mind that you must not press " « " after pressing a letter key«
If you do so by mistake, press Ctrl-Q and repeat step 3.
4. Type ST2D « to bring up the 2D parameter screen.

Adjust SI and NE (only values of 2ⁿ are allowed here) and repeat step 4 until the Hz/Pt resolutions are between 3 and 6 in F1 and between 10 and 20 in F2.
5. Type EXPT« to display the duration of the experiment.

If the duration is too long, decrease D1 (but not below "1") or NE (decrease not by more than 25%, even numbers only) and check the duration again until it is acceptable.
It is not advisable to change NS, but if you do, then only in multiples of 8.
6. Check SF1 and SF2. If they are not equal to the ¹³C frequency, start over from step 1!!
7. Change SF1 to the proton frequency (360.13).
8. Write the parameters to the disk: WJ2D filename.2DP «
9. Turn off the sample spinning and increase the "LOCK GAIN" until the lock signal is at the top edge of the screen.
10. Start the experiment: AU XHCORRD.AU «

    Enter the requested filename. The extension ".SER" is required!!

1. Read your CARBON-13 projection: RE filename.extn «     PJ filename.extn «
2. Set the following parameters:

    SI = 1K,        ND0 = 2,         MC2 = M,         REV = Y,      REDF = N,
    WDW1 = S,   WDW2 = S,    SSB1 = 4,         SSB2 = 4,       NOBC = 0
    (LOCOF1 : SI = 4K,    SSB1 = 0,    SSB2 = 4)
    O2 = O11 = the O1 you found for the proton spectrum.
    O1 = the O1 you found for the carbon spectrum.
    SR1 = the SR you found for the proton spectrum.
    SR2 = the SR you found for the carbon spectrum.
    SW1 = half of the SW you found for the proton spectrum.
    SW2 = the SW you found for the carbon spectrum.
3. Set up the C,H correlation experiment: AS COLOC.AU «

This will display the complete microprogram and then (maybe after a few " « ") it will print out the parameters it needs on the LCD display or on the screen.
You can change them one by one by entering the new values. Recommended are:
    D1 = 2           S1 = 0H        P1 = 10.4       D0 = 3U         D3 = 150M
    P2 = 21          P4 = 10.4      P3 = 5.2         D4 = 50M      S2 = 16H (careful !!)
    RD = 0          PW = 0         DE must not be changed!
    NS = 64         DS = 0          P9 = 80          NE = 128   (NE = 512 for LOCOF1)
    IN must not be changed!
Please keep in mind that you must not press " « " after pressing a letter key!
If you do so by mistake, press Ctrl-Q and repeat step 3.
4. Type ST2D « to bring up the 2D parameter screen.

Adjust SI and NE (only values of 2ⁿ are allowed here) and repeat step 4 until the Hz/Pt resolutions are between 3 and 6 in F1 and between 10 and 20 in F2.
(for LOCOF1: ~0.5 Hz in F1 and ~3 Hz in F2)
5. Type EXPT« to display the duration of the experiment.

If the duration is too long, decrease D1 (but not below "1") or NE (decrease not by more than 25%, even numbers only) and check the duration again until it is acceptable.
It is not advisable to change NS, but if you do, then only in multiples of 8.
6. Check SF1 and SF2. If they are not equal to the ¹³C frequency, start over from step 1!!
7. Change SF1 to the proton frequency (360.13).
8. Find out the values for NE and IN and set D3 = (NE * IN)
9. Write the parameters to the disk: WJ2D filename.2DP «
10. Turn off the sample spinning and increase the "LOCK GAIN" to bring up the lock level.
11. Start the experiment: AU COLOC.AU «

    Enter the requested filename. The extension ".SER" is required!!

1. Read your CARBON-13 projection: RE filename.extn « PJ filename.extn «
2. Set the following parameters:

    SI = 4K,      ND0 = 2,         MC2 = M,        REV = N,      REDF = N,
    WDW1 = S,   WDW2 = S,  SSB1 = 3,         SSB2 = 3,        NOBC = 0
    O2 = O11 = the O1 you found for the proton spectrum.
    O1 = the O1 you found for the carbon spectrum.
    SR1 = the O1 you found for the proton spectrum.
    SR2 = the SR you found for the carbon spectrum.
    SW1 = "300"    and   SW2 = the SW you found for the carbon spectrum.
3. Set up the C,H correlation experiment: AS INEPT2D.AU «

This will display the complete microprogram and then (maybe after a few " « ") it will print out the parameters it needs on the LCD display or on the screen.
4. You can change them one by one by entering the new values. Recommended are:

    D1 = 2         S1 = 0H         P1 = 10.4          D2 = 1.7M         P2 = 21
    P4 = 10.4    P3 = 5.2         D0 = 250U        S2 = 16H (careful !!)
    RD = 0        PW = 0          DE must not be changed!
    NS = 32      DS = 2           P9 = 80              NE = 128          IN must not be changed!
Please keep in mind that you must not press " « " after pressing a letter key!
If you do so by mistake, press Ctrl-Q and repeat step 3.
5. Type ST2D « to bring up the 2D parameter screen.

Adjust SI and NE (only values of 2ⁿ are allowed here) and repeat step 4 until the Hz/Pt resolutions are below 2 in F1 and between 5 and 10 in F2.
6. Type EXPT« to display the duration of the experiment.

If the duration is too long, decrease D1 (but not below "1") or NE (decrease not by more than 25%, even numbers only) and check the duration again until it is acceptable.
It is not advisable to change NS, but if you do, then only in multiples of 8.
7. Check SF1 and SF2. If they are not equal to the ¹³C frequency, start over from step 1!!
8. Change SF1 to the proton frequency (360.13).
9. Write the parameters to the disk: WJ2D filename.2DP «
10. Turn off the sample spinning and increase the "LOCK GAIN" until the lock signal is at the top edge of the screen.
11. Start the experiment: AU INEPT2D.AU «

    Enter the requested filename. The extension ".SER" is required!!

1. Read your CARBON-13 projection: RE filename.extn « PJ filename.extn «
2. Set the following parameters:

    SI = 1K,      ND0 = 2,         MC2 = M,         REV = N,      REDF = N,
    WDW1 = S,   WDW2 = S,  SSB1 = 3,         SSB2 = 3,         NOBC = 0
    O1 = O11 = the O1 you found for the carbon spectrum.
    O2 = the O1 you found for the proton spectrum.
    SR2 = the SR you found for the carbon spectrum. SR1 = O1.
    SW2 = the SW you found for the carbon spectrum. SW1 = 0.25 * SW2
Set up the C,H correlation experiment: AS INADSYM.AU «
This will display the complete microprogram and then (maybe after a few " « ") it will print out the parameters it needs on the LCD display or on the screen.
3. You can change them one by one by entering the new values. Recommended are:

    D1 = 5         P9 = 80             S1 = 18H         D3 = 5M         S2 = 16H
    P1 = 5.2      D2 = 6.5M        P2 = 10.4         D0 = 2U          P3 = 7
    RD = 0        PW = 0             DE must not be changed!
    NS = 32      DS = 0
    LO VCLIST.001
        1 = 1, 2 or 4
        2 = EN
    NE = 256             IN must not be changed!
Please keep in mind that you must not press " « " after pressing a letter key!
If you do so by mistake, press Ctrl-Q and repeat step 3.
4. Type ST2D « to bring up the 2D parameter screen.

Adjust SI and NE simultaneously (only values of 2ⁿ are allowed here) and repeat step 4 until the Hz/Pt resolutions are around 20.
5. Type EXPT« to display the duration of one experiment.

Multiply with the value in the VCLIST to get the total duration. If the duration is too long, decrease NE (decrease not by more than 25%, even numbers only) or VC and check the total duration again until it is acceptable. NS must stay "32"!!
6. Check SF1 and SF2. If they are not equal to the ¹³C frequency, start over from step 1!!
7. Write the parameters to the disk: WJ2D filename.2DP «
8. Turn off the sample spinning and increase the "LOCK GAIN"to bring up the lock signal.
9. Start the experiment: AU INADEQUATE.AU «

    Enter the requested filename. The extension ".SER" is required!

1. RE filename.SER «             (read the experimental data)

RJ filename.2DP «              (read the corresponding parameters
PJ filename.2DP «                       for acquisition, plotting
RJ2D filename.2DP «                    and 2D processing)
Do not change the order of these commands!
2. The default processing parameters usually work fine, but sometimes it may be necessary to change the window functions WDW1, WDW2 and their parameters SSB1, SSB2, LB1, LB2, GB1, GB2, TM1, TM2 to get cleaner spectra.
3. Additional steps for TOCSY and NOESYPH only:
1. Set NOBC = 1, type FT«, enter EP and perform a manual phase correction to make all signals negative. Exit the EP mode.
2. Type TY«, enter the values 0PHZ as PC0 and 1PHZ as PC1. Type PZ«.
4. For all experiments type XFB «. Depending on the size of the 2D matrix this step can

take from a few seconds to several minutes. Check the progress on the top of the screen.
5. Additional steps for TOCSY and NOESYPH only:
1. Type AP2D «, press 'Ctrl-B' to see positive and negative levels and move the cursor to a row with well separated signals. Press 'R' to display the row and 'D' or 'I' to de- or increase the row number, until you find the strongest signals.

Write down this row number and memorize the shape of this 1D spectrum.
2. Press 'Esc' and then 'X' to exit.
3. Type RSC.SMX 'row number' « and correct the phase of the spectrum, until it looks exactly like the row in AP2D.
4. Type TY«, enter the values 0PHZ as PC0 and 1PHZ as PC1. Type PZ«.
6. For TOCSY only:         Type XF1P« and then SYM«

For NOESYPH only:    Type XF1P« and then SYMA«
For JRES only:             Type TILT« and then SYMJ«
For COSY and INADEQUATE only:     Type SYMA«.
7. Type AP2D« and adjust the intensity with the vertical display buttons. The correct level will display only cross- and diagonalpeaks and as little noise as possible.
8. In case the calibration of one or both of the two dimensions is wrong, press 'P', then '1' or '2' for the F1 or F2 projection and then 'E', Press 'Ctrl- R' to see the whole spectrum.

Calibrate the chemical shift scale with a known signal from the 1D spectrum.
Hit "«" until you are back in the 2D display.
9. Press 'L' and define an expanded region with the wheels A - D. Pressing 'X' will expand. The button combination 'M' / 'Y' defines the plotting region.
10. Press 'Esc' and then 'X' to exit AP2D.

 

1. Type DPO « and answer the following questions: (the bold answers are mandatory)

    Draw X-Axis? Y Offset: 0.2
    Mark Separation: 0.1P for proton, 1.0P for carbon-13
    Mark cm: - 0.2 Draw Y-axis? Y
    Mark Separation in F1 Dimension: 0.1P for proton, 1.0P for carbon-13
    Parameters? Y for homonuclear, N for heteronuclear experiments
    Parameters in upper left corner? N
    Rotate? N
    Title? Y
    > (enter the title here, up to 80 characters)
    Center Title? N
2. Set MAXX = 25, MAXY = 17, X0 = 0, Y0 = 0, CY = 15
3. Set CX = 15, if the F1 and F2 dimensions are symmetrical

    (COSY, NOESYPH, TOCSY, INADEQUATE)
or set CX = 18 for experiments with non-symmetrical chemical shift axes.
4. Start the plotting sequence:

-    for COSY and TOCSY: CP2P «
        F1 Projection: (filename of the 1D proton spectrum)
        F2 Projection: (filename of the 1D proton spectrum)
        Number of Levels: (up to 7)
        Number of Pens: (up to 7)
        Plot Frame? Y
        Grid wanted? N
-    for C,H correlation and COLOC: CP2P «
        F1 Projection: (filename of the 1D proton spectrum)
        F2 Projection: (filename of the 1D carbon spectrum)
        Number of Levels: (up to 7)
        Number of Pens: 1
        Plot Frame? Y
        Grid wanted? N
-    for NOESYPH: C2PB «
        F1 Projection: (filename of the 1D proton spectrum)
        F2 Projection: (filename of the 1D proton spectrum)
        Which levels to plot: 0 (zero)
        Pen no. for positive levels: 1 (use a black pen in position 1)
        Pen no. for negative levels: 2 (use a different color in position 2)
        Number of Levels: (up to 7)
        Plot Frame? Y
        Grid wanted? N
-    for INADEQUATE and JRES: CPLP «
        F2 Projection: (filename of the 1D spectrum)
        Number of Levels: (up to 7)
        Number of Pens: up to 7
        Plot Frame? Y
        Grid wanted? N
-    for JRES (alternate method):
        Set CX = 10, CY = 0, MAXY = 15 and customize DPO.
        Enter AP2D« and print selected columns:
            press 'C' to select a column
            'I' and 'D' to step through the slices to find the best trace
            'E' to enter the EP mode
            'Ctrl-R' to display the whole spectrum
            'X' to plot
(C)1998    Jürgen Schulte