[Anion Transport Inhibitors] [Concentration Dependence] [Title page]
Results and Discussion: Temperature dependence of anion exchange
The temperature dependence of the chloride-bicarbonate exchange was examined by measuring the SPQ fluorescence time course at four temperatures from 4.5 C to 35 C. An Arrhenius plot of the results (Figure 6) gives an activation energy of 17 ± 2 kcal/mole. Although our data do not define a transition temperature for chloride-bicarbonate exchange (e.g., 17 C; 16), the activation energy is similar to the value of 19 kcal/mole reported previously when only a single activation energy was assumed (6).
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| Figure 6. Temperature dependence of anion exchange. The chloride-bicarbonate exchange rate is in s-1. The line is a fit of the Arrhenius equation to the data with activation energy 17 ± 2 kcal/mole. Data were obtained with a chloride gradient of -30 mM. |
[Anion Transport Inhibitors] [Concentration Dependence] [Title page]